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Protocols.. 1 mM fluoride (PMSF; from 250 fresh mM in 95% solution ethanol). 2 mM fluoride, 1 dithiothreitol, mM mM. gml 250 for fluoride 60 on ice min and then. Nonidet 0.5% P-40, 1 and mM (PMSF). fluoride Protein was to 100 glane, normalized

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